Pyruvic acid egg medium for tubercle bacilli

Pyruvic acid egg medium for tubercle bacilli.

Sputum culture for tubercle bacilli on a solid egg medium containing glycerol was compared with culture on a similar medium containing pyruvic acid and no glycerol. Tubercle bacilli from the sputum of five out of 99 patients grew on pyruvic acid medium but not on glycerol medium. The addition of pyruvic acid is therefore essential when an egg medium is used for the cultural diagnosis of tubercu...

A Simple Medium for Growth of Tubercle Bacilli *

Blood as a medium for the cultivation of tubercle bacilli was first employed successfully by Koch.' Pryce2 in 1941 reported the use of sputum film cultures suspended in lysed blood, and suggested this technique for pus and caseous material. The medium used in the present study consists of citrated human blood lysed with equal parts of a one per cent solution of saponin in water, to which glycer...

Comparison of solid and liquid medium sensitivity tests of tubercle bacilli to para-aminosalicylic acid.

In the treatment of tuberculosis para-aminosalicylic acid (P.A.S.) is now chiefly used in combination with streptomycin or isoniazid with the aim of preventing or delaying the emergence of drug-resistant strains of tubercle bacilli (Medical Research Council, 1950, 1953c). Such combined therapy is only effective in preventing drug resistance when the strains obtained before the start of treatmen...

Stomach-Wash Examinations for Tubercle Bacilli

Improved sensitivity of direct microscopy for acid-fast bacilli: sedimentation as an alternative to centrifugation for concentration of tubercle bacilli.

There is a great need for improved methods for the diagnosis of tuberculosis by techniques that are appropriate for control programs in low-income countries. Liquefaction of sputum with sodium hypochlorite followed by concentration of bacilli through overnight sedimentation significantly increases the sensitivity of direct microscopy, and this method could be an alternative for diagnostic cente...